1 × te buffer (Sangon Biotech)
90
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Sangon Biotech
1 × te buffer
1 × Te Buffer, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1%C3%97+te+buffer/1+%C3%97+te+buffer/pm40662760-36-13-64
Average 90 stars, based on 1 article reviews
1 × Te Buffer, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1%C3%97+te+buffer/1+%C3%97+te+buffer/pm40662760-36-13-64
Average 90 stars, based on 1 article reviews
1 × te buffer - by Bioz Stars,
2026-10
90/100 stars
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Sequencing:Article Title: A fluorometric lead(II) assay by using a DNA dendrimer as a carrier for the immobilization of the signal probe. Article Snippet: A DNA dendrimer was constructed by combination of rolling circle amplification (RCA) and hybridization chain reaction (HCR).. A fluorescence resonance energy transfer (FRET) pair consisting of 6-carboxyfluorescein (FAM) and 5carboxytetramethylrhodamine (TAMRA) was then created as a signaling probe for the ultrasensitive detection of Pb.. Firstly, the DNAzyme released by Pb-induced recycling amplification as a primer induces RCA to open two different hairpins for generating repeated “Y” structures. Article Title: Mutually Activated Dual-Exponential Amplification DNA Machine Enhances Robust CRISPR/Cas12a Feedback Propagation for Ultrasensitive miRNA Detection. Article Snippet: Breast cancer (BC) remains a critical global health challenge, necessitating ultrasensitive methods for detecting biomarkers such as miR-155, a key regulator in BC progression.. Here, we present a mutually activated dual-exponential amplification DNA machine (MADEA-DNA machine) for ultrasensitive miR-155 detection.. This system integrates exponential rolling circle amplification (E-RCA) and autocatalytic incremental strand displacement amplification (AI-SDA), driven by a bidirectional activation mechanism. Article Title: Ultra-fast, sensitive and low-cost real-time PCR system for nucleic acid detection. Article Snippet: Nucleic acid detection directly identifies the presence of pathogenic microorganisms and has various advantages, such as high sensitivity, commendable specificity and a short window period, and has been widely used in many fields, such as early tumor screening, prenatal diagnosis and infectious disease detection.. Real-time PCR (polymerase chain reaction) is the most commonly used method for nucleic acid detection in clinical practice, but it always takes about 1–3 hours, severely limiting its application in particular scenarios such as emergency testing, large-scale testing and on-site testing.. To solve the timeconsuming problem, a real-time PCR system based on multiple temperature zones was proposed, which realized the speed of temperature change of biological reagents from 2–4 °C s−1 to 13.33 °C s−1. Article Title: Target-Induced Electrochemical Sensor Based on Foldable Aptamer and MoS 2 @MWCNTs-PEI for Enhanced Detection of AFB1 in Peanuts. Article Snippet: Herein, a sensitive and selective electrochemical sensor based on aptamer folding was constructed to detect aflatoxin B1 (AFB1) in peanuts.. Specifically, polyethyleniminefunctionalized multiwalled carbon nanotubes modified with molybdenum disulfide (MoS2@MWCNTs−PEI) were used as the electrode matrix to enable a large specific surface area, which were characterized by the Randles−Sevcik equation.. Additionally, AuNPs were used to immobilize the aptamer via the Au−S covalent bond and provide a favorable microenvironment for signal enhancement. Article Title: The Mechanism of Overflow Amplitude in Nanopore Experiments and Its Application in Molecule Detection. Article Snippet: The investigation of abnormal experimental phenomena observed in nanopore research improves our understanding of nanopores.. In this article, we report and explore the unusual phenomenon that the amplitude of current blockage decreases beyond zero baseline (overflow amplitudes), which was observed in the translocation behavior of 100 bp double-stranded DNA molecules through SiNx nanopores.. In our experiments, the overflow amplitude decreases with the increase of salt concentration and also decreases when the dwell time is shortened as the normalized amplitude of the overflow current showed a reduction with the increase of voltage. Article Title: Dual miRNAs Imaging Platform Based on HRCA-Cas12a by Replacing PAM with Bubble to Reduce False Positive Article Snippet: The sequence of oligonucleotides used in this work .....................................S15 S3 Experimental section Materials and chemicals T4 DNA ligase, T4 Polynucleotide Kinase, Article Title: Accurate Isolation of Circulating Tumor Cells via a Heterovalent DNA Framework Recognition Element-Functionalized Microfluidic Chip. 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Article Snippet: Nucleic acid detection directly identifies the presence of pathogenic microorganisms and has various advantages, such as high sensitivity, commendable specificity and a short window period, and has been widely used in many fields, such as early tumor screening, prenatal diagnosis and infectious disease detection.. Real-time PCR (polymerase chain reaction) is the most commonly used method for nucleic acid detection in clinical practice, but it always takes about 1–3 hours, severely limiting its application in particular scenarios such as emergency testing, large-scale testing and on-site testing.. To solve the timeconsuming problem, a real-time PCR system based on multiple temperature zones was proposed, which realized the speed of temperature change of biological reagents from 2–4 °C s−1 to 13.33 °C s−1. Article Title: Target-Induced Electrochemical Sensor Based on Foldable Aptamer and MoS 2 @MWCNTs-PEI for Enhanced Detection of AFB1 in Peanuts. Article Snippet: Herein, a sensitive and selective electrochemical sensor based on aptamer folding was constructed to detect aflatoxin B1 (AFB1) in peanuts.. Specifically, polyethyleniminefunctionalized multiwalled carbon nanotubes modified with molybdenum disulfide (MoS2@MWCNTs−PEI) were used as the electrode matrix to enable a large specific surface area, which were characterized by the Randles−Sevcik equation.. Additionally, AuNPs were used to immobilize the aptamer via the Au−S covalent bond and provide a favorable microenvironment for signal enhancement. Article Title: The Mechanism of Overflow Amplitude in Nanopore Experiments and Its Application in Molecule Detection. Article Snippet: The investigation of abnormal experimental phenomena observed in nanopore research improves our understanding of nanopores.. In this article, we report and explore the unusual phenomenon that the amplitude of current blockage decreases beyond zero baseline (overflow amplitudes), which was observed in the translocation behavior of 100 bp double-stranded DNA molecules through SiNx nanopores.. In our experiments, the overflow amplitude decreases with the increase of salt concentration and also decreases when the dwell time is shortened as the normalized amplitude of the overflow current showed a reduction with the increase of voltage. Article Title: Dual miRNAs Imaging Platform Based on HRCA-Cas12a by Replacing PAM with Bubble to Reduce False Positive Article Snippet: The sequence of oligonucleotides used in this work .....................................S15 S3 Experimental section Materials and chemicals T4 DNA ligase, T4 Polynucleotide Kinase, Article Title: Accurate Isolation of Circulating Tumor Cells via a Heterovalent DNA Framework Recognition Element-Functionalized Microfluidic Chip. Article Snippet: 1× TE buffer, 10× TM buffer, 10× TBE Premixed Powder and DNA Marker were purchased from Article Title: Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification Article Snippet: A viral DNA/RNA extraction kit (magnetic bead method, GenMagBio, Changzhou, China) was used to extract the AD169 strain of HCMV virus that was cultured by Polymerase Chain Reaction:Article Title: A fluorometric lead(II) assay by using a DNA dendrimer as a carrier for the immobilization of the signal probe. Article Snippet: A DNA dendrimer was constructed by combination of rolling circle amplification (RCA) and hybridization chain reaction (HCR).. A fluorescence resonance energy transfer (FRET) pair consisting of 6-carboxyfluorescein (FAM) and 5carboxytetramethylrhodamine (TAMRA) was then created as a signaling probe for the ultrasensitive detection of Pb.. Firstly, the DNAzyme released by Pb-induced recycling amplification as a primer induces RCA to open two different hairpins for generating repeated “Y” structures. Article Title: Mutually Activated Dual-Exponential Amplification DNA Machine Enhances Robust CRISPR/Cas12a Feedback Propagation for Ultrasensitive miRNA Detection. Article Snippet: Breast cancer (BC) remains a critical global health challenge, necessitating ultrasensitive methods for detecting biomarkers such as miR-155, a key regulator in BC progression.. Here, we present a mutually activated dual-exponential amplification DNA machine (MADEA-DNA machine) for ultrasensitive miR-155 detection.. This system integrates exponential rolling circle amplification (E-RCA) and autocatalytic incremental strand displacement amplification (AI-SDA), driven by a bidirectional activation mechanism. Article Title: Ultra-fast, sensitive and low-cost real-time PCR system for nucleic acid detection. Article Snippet: Nucleic acid detection directly identifies the presence of pathogenic microorganisms and has various advantages, such as high sensitivity, commendable specificity and a short window period, and has been widely used in many fields, such as early tumor screening, prenatal diagnosis and infectious disease detection.. Real-time PCR (polymerase chain reaction) is the most commonly used method for nucleic acid detection in clinical practice, but it always takes about 1–3 hours, severely limiting its application in particular scenarios such as emergency testing, large-scale testing and on-site testing.. To solve the timeconsuming problem, a real-time PCR system based on multiple temperature zones was proposed, which realized the speed of temperature change of biological reagents from 2–4 °C s−1 to 13.33 °C s−1. Article Title: Target-Induced Electrochemical Sensor Based on Foldable Aptamer and MoS 2 @MWCNTs-PEI for Enhanced Detection of AFB1 in Peanuts. Article Snippet: Herein, a sensitive and selective electrochemical sensor based on aptamer folding was constructed to detect aflatoxin B1 (AFB1) in peanuts.. Specifically, polyethyleniminefunctionalized multiwalled carbon nanotubes modified with molybdenum disulfide (MoS2@MWCNTs−PEI) were used as the electrode matrix to enable a large specific surface area, which were characterized by the Randles−Sevcik equation.. Additionally, AuNPs were used to immobilize the aptamer via the Au−S covalent bond and provide a favorable microenvironment for signal enhancement. Article Title: The Mechanism of Overflow Amplitude in Nanopore Experiments and Its Application in Molecule Detection. Article Snippet: The investigation of abnormal experimental phenomena observed in nanopore research improves our understanding of nanopores.. In this article, we report and explore the unusual phenomenon that the amplitude of current blockage decreases beyond zero baseline (overflow amplitudes), which was observed in the translocation behavior of 100 bp double-stranded DNA molecules through SiNx nanopores.. In our experiments, the overflow amplitude decreases with the increase of salt concentration and also decreases when the dwell time is shortened as the normalized amplitude of the overflow current showed a reduction with the increase of voltage. Article Title: Dual miRNAs Imaging Platform Based on HRCA-Cas12a by Replacing PAM with Bubble to Reduce False Positive Article Snippet: The sequence of oligonucleotides used in this work .....................................S15 S3 Experimental section Materials and chemicals T4 DNA ligase, T4 Polynucleotide Kinase, Article Title: Accurate Isolation of Circulating Tumor Cells via a Heterovalent DNA Framework Recognition Element-Functionalized Microfluidic Chip. Article Snippet: 1× TE buffer, 10× TM buffer, 10× TBE Premixed Powder and DNA Marker were purchased from Article Title: Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification Article Snippet: A viral DNA/RNA extraction kit (magnetic bead method, GenMagBio, Changzhou, China) was used to extract the AD169 strain of HCMV virus that was cultured by Marker:Article Title: A fluorometric lead(II) assay by using a DNA dendrimer as a carrier for the immobilization of the signal probe. Article Snippet: A DNA dendrimer was constructed by combination of rolling circle amplification (RCA) and hybridization chain reaction (HCR).. A fluorescence resonance energy transfer (FRET) pair consisting of 6-carboxyfluorescein (FAM) and 5carboxytetramethylrhodamine (TAMRA) was then created as a signaling probe for the ultrasensitive detection of Pb.. Firstly, the DNAzyme released by Pb-induced recycling amplification as a primer induces RCA to open two different hairpins for generating repeated “Y” structures. Article Title: Mutually Activated Dual-Exponential Amplification DNA Machine Enhances Robust CRISPR/Cas12a Feedback Propagation for Ultrasensitive miRNA Detection. Article Snippet: Breast cancer (BC) remains a critical global health challenge, necessitating ultrasensitive methods for detecting biomarkers such as miR-155, a key regulator in BC progression.. Here, we present a mutually activated dual-exponential amplification DNA machine (MADEA-DNA machine) for ultrasensitive miR-155 detection.. This system integrates exponential rolling circle amplification (E-RCA) and autocatalytic incremental strand displacement amplification (AI-SDA), driven by a bidirectional activation mechanism. Article Title: Ultra-fast, sensitive and low-cost real-time PCR system for nucleic acid detection. Article Snippet: Nucleic acid detection directly identifies the presence of pathogenic microorganisms and has various advantages, such as high sensitivity, commendable specificity and a short window period, and has been widely used in many fields, such as early tumor screening, prenatal diagnosis and infectious disease detection.. Real-time PCR (polymerase chain reaction) is the most commonly used method for nucleic acid detection in clinical practice, but it always takes about 1–3 hours, severely limiting its application in particular scenarios such as emergency testing, large-scale testing and on-site testing.. To solve the timeconsuming problem, a real-time PCR system based on multiple temperature zones was proposed, which realized the speed of temperature change of biological reagents from 2–4 °C s−1 to 13.33 °C s−1. Article Title: Target-Induced Electrochemical Sensor Based on Foldable Aptamer and MoS 2 @MWCNTs-PEI for Enhanced Detection of AFB1 in Peanuts. Article Snippet: Herein, a sensitive and selective electrochemical sensor based on aptamer folding was constructed to detect aflatoxin B1 (AFB1) in peanuts.. Specifically, polyethyleniminefunctionalized multiwalled carbon nanotubes modified with molybdenum disulfide (MoS2@MWCNTs−PEI) were used as the electrode matrix to enable a large specific surface area, which were characterized by the Randles−Sevcik equation.. Additionally, AuNPs were used to immobilize the aptamer via the Au−S covalent bond and provide a favorable microenvironment for signal enhancement. Article Title: The Mechanism of Overflow Amplitude in Nanopore Experiments and Its Application in Molecule Detection. Article Snippet: The investigation of abnormal experimental phenomena observed in nanopore research improves our understanding of nanopores.. In this article, we report and explore the unusual phenomenon that the amplitude of current blockage decreases beyond zero baseline (overflow amplitudes), which was observed in the translocation behavior of 100 bp double-stranded DNA molecules through SiNx nanopores.. In our experiments, the overflow amplitude decreases with the increase of salt concentration and also decreases when the dwell time is shortened as the normalized amplitude of the overflow current showed a reduction with the increase of voltage. Article Title: Dual miRNAs Imaging Platform Based on HRCA-Cas12a by Replacing PAM with Bubble to Reduce False Positive Article Snippet: The sequence of oligonucleotides used in this work .....................................S15 S3 Experimental section Materials and chemicals T4 DNA ligase, T4 Polynucleotide Kinase, Article Title: Accurate Isolation of Circulating Tumor Cells via a Heterovalent DNA Framework Recognition Element-Functionalized Microfluidic Chip. Article Snippet: 1× TE buffer, 10× TM buffer, 10× TBE Premixed Powder and DNA Marker were purchased from Article Title: Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification Article Snippet: A viral DNA/RNA extraction kit (magnetic bead method, GenMagBio, Changzhou, China) was used to extract the AD169 strain of HCMV virus that was cultured by Extraction:Article Title: A fluorometric lead(II) assay by using a DNA dendrimer as a carrier for the immobilization of the signal probe. Article Snippet: A DNA dendrimer was constructed by combination of rolling circle amplification (RCA) and hybridization chain reaction (HCR).. A fluorescence resonance energy transfer (FRET) pair consisting of 6-carboxyfluorescein (FAM) and 5carboxytetramethylrhodamine (TAMRA) was then created as a signaling probe for the ultrasensitive detection of Pb.. Firstly, the DNAzyme released by Pb-induced recycling amplification as a primer induces RCA to open two different hairpins for generating repeated “Y” structures. Article Title: Mutually Activated Dual-Exponential Amplification DNA Machine Enhances Robust CRISPR/Cas12a Feedback Propagation for Ultrasensitive miRNA Detection. Article Snippet: Breast cancer (BC) remains a critical global health challenge, necessitating ultrasensitive methods for detecting biomarkers such as miR-155, a key regulator in BC progression.. Here, we present a mutually activated dual-exponential amplification DNA machine (MADEA-DNA machine) for ultrasensitive miR-155 detection.. This system integrates exponential rolling circle amplification (E-RCA) and autocatalytic incremental strand displacement amplification (AI-SDA), driven by a bidirectional activation mechanism. Article Title: Ultra-fast, sensitive and low-cost real-time PCR system for nucleic acid detection. Article Snippet: Nucleic acid detection directly identifies the presence of pathogenic microorganisms and has various advantages, such as high sensitivity, commendable specificity and a short window period, and has been widely used in many fields, such as early tumor screening, prenatal diagnosis and infectious disease detection.. Real-time PCR (polymerase chain reaction) is the most commonly used method for nucleic acid detection in clinical practice, but it always takes about 1–3 hours, severely limiting its application in particular scenarios such as emergency testing, large-scale testing and on-site testing.. To solve the timeconsuming problem, a real-time PCR system based on multiple temperature zones was proposed, which realized the speed of temperature change of biological reagents from 2–4 °C s−1 to 13.33 °C s−1. Article Title: Target-Induced Electrochemical Sensor Based on Foldable Aptamer and MoS 2 @MWCNTs-PEI for Enhanced Detection of AFB1 in Peanuts. Article Snippet: Herein, a sensitive and selective electrochemical sensor based on aptamer folding was constructed to detect aflatoxin B1 (AFB1) in peanuts.. Specifically, polyethyleniminefunctionalized multiwalled carbon nanotubes modified with molybdenum disulfide (MoS2@MWCNTs−PEI) were used as the electrode matrix to enable a large specific surface area, which were characterized by the Randles−Sevcik equation.. Additionally, AuNPs were used to immobilize the aptamer via the Au−S covalent bond and provide a favorable microenvironment for signal enhancement. Article Title: The Mechanism of Overflow Amplitude in Nanopore Experiments and Its Application in Molecule Detection. Article Snippet: The investigation of abnormal experimental phenomena observed in nanopore research improves our understanding of nanopores.. In this article, we report and explore the unusual phenomenon that the amplitude of current blockage decreases beyond zero baseline (overflow amplitudes), which was observed in the translocation behavior of 100 bp double-stranded DNA molecules through SiNx nanopores.. In our experiments, the overflow amplitude decreases with the increase of salt concentration and also decreases when the dwell time is shortened as the normalized amplitude of the overflow current showed a reduction with the increase of voltage. Article Title: Dual miRNAs Imaging Platform Based on HRCA-Cas12a by Replacing PAM with Bubble to Reduce False Positive Article Snippet: The sequence of oligonucleotides used in this work .....................................S15 S3 Experimental section Materials and chemicals T4 DNA ligase, T4 Polynucleotide Kinase, Article Title: Accurate Isolation of Circulating Tumor Cells via a Heterovalent DNA Framework Recognition Element-Functionalized Microfluidic Chip. Article Snippet: 1× TE buffer, 10× TM buffer, 10× TBE Premixed Powder and DNA Marker were purchased from Article Title: Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification Article Snippet: A viral DNA/RNA extraction kit (magnetic bead method, GenMagBio, Changzhou, China) was used to extract the AD169 strain of HCMV virus that was cultured by Virus:Article Title: A fluorometric lead(II) assay by using a DNA dendrimer as a carrier for the immobilization of the signal probe. Article Snippet: A DNA dendrimer was constructed by combination of rolling circle amplification (RCA) and hybridization chain reaction (HCR).. A fluorescence resonance energy transfer (FRET) pair consisting of 6-carboxyfluorescein (FAM) and 5carboxytetramethylrhodamine (TAMRA) was then created as a signaling probe for the ultrasensitive detection of Pb.. Firstly, the DNAzyme released by Pb-induced recycling amplification as a primer induces RCA to open two different hairpins for generating repeated “Y” structures. Article Title: Mutually Activated Dual-Exponential Amplification DNA Machine Enhances Robust CRISPR/Cas12a Feedback Propagation for Ultrasensitive miRNA Detection. Article Snippet: Breast cancer (BC) remains a critical global health challenge, necessitating ultrasensitive methods for detecting biomarkers such as miR-155, a key regulator in BC progression.. Here, we present a mutually activated dual-exponential amplification DNA machine (MADEA-DNA machine) for ultrasensitive miR-155 detection.. This system integrates exponential rolling circle amplification (E-RCA) and autocatalytic incremental strand displacement amplification (AI-SDA), driven by a bidirectional activation mechanism. Article Title: Ultra-fast, sensitive and low-cost real-time PCR system for nucleic acid detection. Article Snippet: Nucleic acid detection directly identifies the presence of pathogenic microorganisms and has various advantages, such as high sensitivity, commendable specificity and a short window period, and has been widely used in many fields, such as early tumor screening, prenatal diagnosis and infectious disease detection.. Real-time PCR (polymerase chain reaction) is the most commonly used method for nucleic acid detection in clinical practice, but it always takes about 1–3 hours, severely limiting its application in particular scenarios such as emergency testing, large-scale testing and on-site testing.. To solve the timeconsuming problem, a real-time PCR system based on multiple temperature zones was proposed, which realized the speed of temperature change of biological reagents from 2–4 °C s−1 to 13.33 °C s−1. Article Title: Target-Induced Electrochemical Sensor Based on Foldable Aptamer and MoS 2 @MWCNTs-PEI for Enhanced Detection of AFB1 in Peanuts. Article Snippet: Herein, a sensitive and selective electrochemical sensor based on aptamer folding was constructed to detect aflatoxin B1 (AFB1) in peanuts.. Specifically, polyethyleniminefunctionalized multiwalled carbon nanotubes modified with molybdenum disulfide (MoS2@MWCNTs−PEI) were used as the electrode matrix to enable a large specific surface area, which were characterized by the Randles−Sevcik equation.. Additionally, AuNPs were used to immobilize the aptamer via the Au−S covalent bond and provide a favorable microenvironment for signal enhancement. Article Title: The Mechanism of Overflow Amplitude in Nanopore Experiments and Its Application in Molecule Detection. Article Snippet: The investigation of abnormal experimental phenomena observed in nanopore research improves our understanding of nanopores.. In this article, we report and explore the unusual phenomenon that the amplitude of current blockage decreases beyond zero baseline (overflow amplitudes), which was observed in the translocation behavior of 100 bp double-stranded DNA molecules through SiNx nanopores.. In our experiments, the overflow amplitude decreases with the increase of salt concentration and also decreases when the dwell time is shortened as the normalized amplitude of the overflow current showed a reduction with the increase of voltage. Article Title: Dual miRNAs Imaging Platform Based on HRCA-Cas12a by Replacing PAM with Bubble to Reduce False Positive Article Snippet: The sequence of oligonucleotides used in this work .....................................S15 S3 Experimental section Materials and chemicals T4 DNA ligase, T4 Polynucleotide Kinase, Article Title: Accurate Isolation of Circulating Tumor Cells via a Heterovalent DNA Framework Recognition Element-Functionalized Microfluidic Chip. Article Snippet: 1× TE buffer, 10× TM buffer, 10× TBE Premixed Powder and DNA Marker were purchased from Article Title: Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification Article Snippet: A viral DNA/RNA extraction kit (magnetic bead method, GenMagBio, Changzhou, China) was used to extract the AD169 strain of HCMV virus that was cultured by Cell Culture:Article Title: A fluorometric lead(II) assay by using a DNA dendrimer as a carrier for the immobilization of the signal probe. Article Snippet: A DNA dendrimer was constructed by combination of rolling circle amplification (RCA) and hybridization chain reaction (HCR).. A fluorescence resonance energy transfer (FRET) pair consisting of 6-carboxyfluorescein (FAM) and 5carboxytetramethylrhodamine (TAMRA) was then created as a signaling probe for the ultrasensitive detection of Pb.. Firstly, the DNAzyme released by Pb-induced recycling amplification as a primer induces RCA to open two different hairpins for generating repeated “Y” structures. Article Title: Mutually Activated Dual-Exponential Amplification DNA Machine Enhances Robust CRISPR/Cas12a Feedback Propagation for Ultrasensitive miRNA Detection. Article Snippet: Breast cancer (BC) remains a critical global health challenge, necessitating ultrasensitive methods for detecting biomarkers such as miR-155, a key regulator in BC progression.. Here, we present a mutually activated dual-exponential amplification DNA machine (MADEA-DNA machine) for ultrasensitive miR-155 detection.. This system integrates exponential rolling circle amplification (E-RCA) and autocatalytic incremental strand displacement amplification (AI-SDA), driven by a bidirectional activation mechanism. Article Title: Ultra-fast, sensitive and low-cost real-time PCR system for nucleic acid detection. Article Snippet: Nucleic acid detection directly identifies the presence of pathogenic microorganisms and has various advantages, such as high sensitivity, commendable specificity and a short window period, and has been widely used in many fields, such as early tumor screening, prenatal diagnosis and infectious disease detection.. Real-time PCR (polymerase chain reaction) is the most commonly used method for nucleic acid detection in clinical practice, but it always takes about 1–3 hours, severely limiting its application in particular scenarios such as emergency testing, large-scale testing and on-site testing.. To solve the timeconsuming problem, a real-time PCR system based on multiple temperature zones was proposed, which realized the speed of temperature change of biological reagents from 2–4 °C s−1 to 13.33 °C s−1. Article Title: Target-Induced Electrochemical Sensor Based on Foldable Aptamer and MoS 2 @MWCNTs-PEI for Enhanced Detection of AFB1 in Peanuts. Article Snippet: Herein, a sensitive and selective electrochemical sensor based on aptamer folding was constructed to detect aflatoxin B1 (AFB1) in peanuts.. Specifically, polyethyleniminefunctionalized multiwalled carbon nanotubes modified with molybdenum disulfide (MoS2@MWCNTs−PEI) were used as the electrode matrix to enable a large specific surface area, which were characterized by the Randles−Sevcik equation.. Additionally, AuNPs were used to immobilize the aptamer via the Au−S covalent bond and provide a favorable microenvironment for signal enhancement. Article Title: The Mechanism of Overflow Amplitude in Nanopore Experiments and Its Application in Molecule Detection. Article Snippet: The investigation of abnormal experimental phenomena observed in nanopore research improves our understanding of nanopores.. In this article, we report and explore the unusual phenomenon that the amplitude of current blockage decreases beyond zero baseline (overflow amplitudes), which was observed in the translocation behavior of 100 bp double-stranded DNA molecules through SiNx nanopores.. In our experiments, the overflow amplitude decreases with the increase of salt concentration and also decreases when the dwell time is shortened as the normalized amplitude of the overflow current showed a reduction with the increase of voltage. Article Title: Dual miRNAs Imaging Platform Based on HRCA-Cas12a by Replacing PAM with Bubble to Reduce False Positive Article Snippet: The sequence of oligonucleotides used in this work .....................................S15 S3 Experimental section Materials and chemicals T4 DNA ligase, T4 Polynucleotide Kinase, Article Title: Accurate Isolation of Circulating Tumor Cells via a Heterovalent DNA Framework Recognition Element-Functionalized Microfluidic Chip. Article Snippet: 1× TE buffer, 10× TM buffer, 10× TBE Premixed Powder and DNA Marker were purchased from Article Title: Ultrafast Microfluidic PCR Thermocycler for Nucleic Acid Amplification Article Snippet: A viral DNA/RNA extraction kit (magnetic bead method, GenMagBio, Changzhou, China) was used to extract the AD169 strain of HCMV virus that was cultured by |